The 2000 Nine Foundation Diddy Kong Racing League was a video game league where 5 video game players competed in a league to see who was the best at the Nintendo 64 racing game Diddy Kong Racing. This was the follow up to the 1999 Nine Foundation Diddy Kong Racing League.

The league consisted of 25 games where each player would play each other in the five trophy races. Nine Corporation (then Nine Foundation) sponsored the event.

Antonio Matterson was crowned the champion of the 2000 Nine Foundation Diddy Kong Racing League.